Comparative mass spectrometric analyses of Photofrin oligomers by fast atom bombardment mass spectrometry, UV and IR matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry and laser desorption/jet-cooling photoionization mass spectrometry.
نویسندگان
چکیده
Photofrin (porfimer sodium) is a porphyrin derivative used in the treatment of a variety of cancers by photodynamic therapy. This oligomer complex and a variety of porphyrin monomers, dimers and trimers were analyzed with five different mass spectral ionization techniques: fast atom bombardment, UV and IR matrix-assisted laser desorption/ionization, electrospray ionization, and laser desorption/jet-cooling photoionization. All five approaches resulted in very similar oligomer distributions with an average oligomer length of 2.7 +/- 0.1 porphyrin units. In addition to the Photofrin analysis, this study provides a side-by-side comparison of the spectra for the five different mass spectrometric techniques.
منابع مشابه
Liquid Secondary Ion Mass Spectrometry
Liquid secondary-ion mass spectrometry (liquid SIMS) is an obsolescent ionization technique that revolutionized the analyses of large biological molecules in the 1980s. The term includes fast atom bombardment mass spectrometry (FAB-MS). The technique has been largely superseded by electrospray ionization. This account will cover the history of the technique, the principles of the operation, and...
متن کاملMALDI-TOF MS Coupled with Collision-Induced Dissociation (CID) Measurements of Poly(methyl methacrylate)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is one of themost fundamental analysis techniques for proteins, peptides, and synthetic polymers to date. Along with field desorption (FD), fast atom bombardment (FAB), laser desorption coupled with time-of-flight (LD-TOF), secondary ion mass spectrometry coupled with time-of-flight (SIMS-TOF), and electr...
متن کاملDirect Analysis of Triterpenes from High-Salt Fermented Cucumbers Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI).
High-salt samples present a challenge to mass spectrometry (MS) analysis, particularly when electrospray ionization (ESI) is used, requiring extensive sample preparation steps such as desalting, extraction, and purification. In this study, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to a Q Exactive Plus mass spectrometer was used to directly analyze 50...
متن کاملMass spectrometric analysis of mercury incorporation into proteins for X-ray diffraction phase determination.
Heavy-atom incorporation is an essential and often rate-limiting step in the determination of phases for X-ray diffraction studies of protein structures. Until the present, there has been no practical method (short of the X-ray diffraction experiment itself) to judge the success and extent of incorporation. Here we show that mass spectrometry is an effective tool for determining the extent of h...
متن کاملDesorption Ionization Mass Spectrometry
Common to all experiments in mass spectrometry is the creation of gas-phase ions. Electron impact (EI) ionization is a familiar method for creating ions from volatile gas-phase molecules (M). In EI ionization, fast-moving electrons remove an electron from the neutral molecule to create the odd-electron molecular ion M`Õ. Another common ionization method is chemical ionization (CI). Here, an ion...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of mass spectrometry : JMS
دوره 34 6 شماره
صفحات -
تاریخ انتشار 1999